Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Random amplified polymorphic DNA observed in Eucalyptus by PCR study with random primers

M Izumi1, E Kojima, M Matsuda

  • 1Biochemistry Section, Iwakuni Research Laboratory of Technology, Nippon Paper Industries Co. Ltd, Yamaguchi, Japan.

Nucleic Acids Symposium Series
|January 1, 1997
PubMed
Summary

This study used random primers and Polymerase Chain Reaction (PCR) to analyze DNA from Eucalyptus species. Researchers identified specific primers that revealed genetic variations within Eucalyptus globlus, E. citriodora, and Maruha Yu-kari.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

All-trans-retinoic acid inhibits tumour growth of malignant pleural mesothelioma in mice.

The European respiratory journal·2009
Same author

Characterization of monoclonal antibodies directed against squamous cell carcinoma antigens: report of the second TD-10 workshop.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine·2009
Same author

[Risk management for congenital heart surgery].

Kyobu geka. The Japanese journal of thoracic surgery·2008
Same author

NOTES transvaginal video-assisted cholecystectomy: first series.

Endoscopy·2008
Same author

Depositional records of plutonium and (137)Cs released from Nagasaki atomic bomb in sediment of Nishiyama reservoir at Nagasaki.

Journal of environmental radioactivity·2008
Same author

Observation of time-dependent CP violation in B0 --> eta'K0 decays and improved measurements of CP asymmetries in B0 --> phiK0, KS0KS0KS0 and B0 --> J/psiK0 decays.

Physical review letters·2007

Area of Science:

  • Plant genetics
  • Molecular biology
  • Biotechnology

Background:

  • Eucalyptus species are vital for timber and essential oils.
  • Understanding genetic diversity is crucial for conservation and breeding programs.
  • Random amplified polymorphic DNA (RAPD) is a useful molecular marker technique.

Purpose of the Study:

  • To evaluate the effectiveness of random primers in detecting DNA polymorphism in Eucalyptus species.
  • To identify specific primers that can differentiate between Eucalyptus globlus, E. citriodora, and E. gunnii (Maruha Yu-kari).

Main Methods:

  • DNA was extracted from three Eucalyptus species: E. globlus, E. citriodora, and Maruha Yu-kari.
  • Polymerase Chain Reaction (PCR) was performed using 40 different random 10-mer primers.

Related Experiment Videos

  • PCR products were analyzed via agarose gel electrophoresis to assess DNA fragment patterns (0.5-3.0 kbp).

    • Main Results:

    • A total of 40 random primers were screened for each Eucalyptus species.
    • Nine primers successfully generated polymorphic DNA profiles for E. globlus.
    • Six primers each revealed polymorphism in E. citriodora and Maruha Yu-kari.

    Conclusions:

    • Random amplified polymorphic DNA (RAPD) markers are effective for assessing genetic diversity in Eucalyptus.
    • Specific primer sets were identified for distinguishing genetic variations within the studied Eucalyptus species.
    • This research provides a foundation for marker-assisted selection in Eucalyptus breeding and conservation.