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Stabilities of internal rU-dG and rG-dT pairs in RNA/DNA hybrids
N Sugimoto1, I Yasumatsu, M Fujimoto
1Department of Chemistry, Faculty of Science, Konan University, Kobe, Japan.
Nucleic Acids Symposium Series
|January 1, 1997
Abstract:
We report stabilities of internal non-Watson-Crick rU-dG and rG-dT pairs in RNA/DNA hybrids. UV melting curves revealed that a hybrid containing internal rG-dT pair had about 2 kcal mol-1 greater stability in free energy change at 37 degrees C than hybrids with other mismatches, rA-dA and rU-dT, at the same position. We discuss why internal rG-dT as well as rU-dG is stable nearly Watson-Crick base pairs rather than the other mismatches.