Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Early maternal separation increases NGF expression in the developing rat hippocampus

F Cirulli1, A Micera, E Alleva

  • 1Section of Behavioral Pathophysiology, Laboratorio di Fisiopatologia di Organo e di Sistema, Istituto Superiore di Sanità, Rome, Italy.

Pharmacology, Biochemistry, and Behavior
|May 20, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intranasal self-administration of high-pressure physiological saline isotonic solution ameliorates dry eye symptoms and tear film composition: a pilot study.

European review for medical and pharmacological sciences·2023
Same author

Antiviral effect of high-pressure nasal stimulation.

European review for medical and pharmacological sciences·2023
Same author

Enhanced presence of serotonin in nasal cavity after autologous stimulation.

European review for medical and pharmacological sciences·2022
Same author

Keratoconus: the possible involvement of inflammatory cytokines in its pathogenesis. An experimental study and review of the literature.

European review for medical and pharmacological sciences·2021
Same author

Acute environmental temperature variation affects brain protein expression, anxiety and explorative behaviour in adult zebrafish.

Scientific reports·2021
Same author

Nerve growth factor serum levels in treatment-resistant schizophrenic patients following electroconvulsive therapy.

La Clinica terapeutica·2020

Early maternal separation in rat pups increases Nerve Growth Factor (NGF) mRNA in the hippocampus. However, NGF protein levels remained unchanged, suggesting complex developmental responses to early life stress.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Molecular Psychiatry

Background:

  • Nerve Growth Factor (NGF) is crucial for the development and survival of central cholinergic neurons.
  • Early life experiences, including maternal separation, can significantly impact neurodevelopment.
  • Understanding how early stress affects neurotrophic factors like NGF is vital for developmental neuroscience.

Purpose of the Study:

  • To investigate the effects of brief maternal separation on Nerve Growth Factor (NGF) expression in neonatal rat pups.
  • To determine if early life stress alters NGF mRNA and protein levels in the hippocampus.

Main Methods:

  • Utilized a maternal separation paradigm with 3-day-old rat pups.
  • Examined NGF mRNA expression using in situ hybridization.

Related Experiment Videos

  • Quantified NGF protein levels via ELISA in the hippocampus.

    • Main Results:

    • Neonatal maternal separation led to increased NGF mRNA expression in the dentate gyrus and hilus of the hippocampus.
    • No significant difference in total hippocampal NGF protein levels was observed between separated and non-separated pups the day after separation.

    Conclusions:

    • Brief early-life manipulations, such as maternal separation, can modulate hippocampal NGF gene expression.
    • The study highlights a dissociation between NGF mRNA and protein responses to early stress, indicating complex regulatory mechanisms.