[Frequency of the HLA-DPB1 allele in the Slovak population]

The polymorphism at the HLA-DPB1 locus was established in 146 unrelated persons. The polymerase chain reaction (PCR) in combination with restriction fragment length polymorphism (RFLP) technique was used. After PCR amplification of the second exon of the HLA-DPB1 locus, the PCR products were digested with seven allele specific endonucleases. The alleles DPB1*0401 (43.29%), DPB1*0402 (20.89%), DPB1*0201 (14.39%) and DPB1*0301 (9.25%) were the most common among 15 DPB1 alleles detected in the tested group. The least frequent alleles were DPB1 *0202, *0601, *1101 and *1501 (0.34%). The comparison with allele frequencies in Austrian and German populations showed no statistically significant differences. (Tab. 2, Ref. 18.)