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Telomerase activity in human gliomas

S Le1, J J Zhu, D C Anthony

  • 1Cold Spring Harbor Laboratory, New York, USA.

Neurosurgery
|May 20, 1998
PubMed
Summary

Telomerase activity is absent in normal brain tissue but present in most gliomas. Its frequency increases with tumor grade, suggesting telomerase may be a tumor marker for astrocytic tumors.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Neuroscience

Background:

  • Telomerase activity is typically undetectable in normal tissues but found in various cancers.
  • This suggests telomerase activation is a key factor in tumorigenesis, potentially alongside oncogene and tumor suppressor gene alterations.

Purpose of the Study:

  • To evaluate telomerase activity in astrocytic gliomas.
  • To correlate telomerase activity with different glioma grades.

Main Methods:

  • Telomere Repeat Amplification Protocol (TRAP) was used to detect telomerase activity.
  • Southern blot hybridization assessed terminal restriction fragment length.

Main Results:

  • Telomerase activity was detected in 33% of low-grade astrocytomas (WHO Grade II), 45% of anaplastic astrocytomas (WHO Grade III), and 89% of glioblastomas multiforme (WHO Grade IV).
  • Activity was also found in 75% of oligodendrogliomas, but not in normal brain specimens.
  • Overall, 72% of glioma samples exhibited telomerase activity.

Conclusions:

  • Telomerase activity is absent in normal brain tissue but prevalent in gliomas.
  • The frequency of telomerase activity correlates positively with the malignancy grade of astrocytic tumors.
  • These findings indicate telomerase activity could serve as a diagnostic marker and its activation may be linked to tumor initiation and progression.

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