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Related Experiment Videos

Nucleosomes in metaphase chromosomes

M H Wigler, R Axel

    Nucleic Acids Research
    |June 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    This study investigated metaphase chromosome structure, comparing it to interphase chromatin. Results show that the basic nucleosome structure is maintained during mitosis, despite morphological changes.

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    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Genetics

    Background:

    • Previous research on metaphase chromosome structure utilized electron micrography, identifying a 10-nm fiber potentially forming the 23-30-nm fiber through higher-order folding.
    • The relationship between metaphase and interphase fibers remained unclear.
    • Recent studies identified nucleosomes as repeating DNA-histone subunits in interphase chromatin.

    Purpose of the Study:

    • To investigate the presence of nucleosomal subunits within metaphase chromosomes.
    • To compare the structural integrity of chromatin during mitosis with interphase.

    Main Methods:

    • Metaphase chromosomes were isolated from mouse L-cells arrested with colchicine.
    • Chromosomes were subjected to digestion with staphylococcal nuclease.

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  • Digestion products were analyzed and compared to those from interphase nuclei.

    • Main Results:

    • Analysis revealed that the basic subunit structure of the chromatin fiber is retained in mitotic chromosomes.
    • Significant morphological alterations occur in the chromatin fiber during mitosis.
    • Comparison of early and limit digestion products indicated structural conservation.

    Conclusions:

    • The fundamental nucleosomal structure of chromatin is preserved during mitosis.
    • Mitotic chromosomes maintain the basic DNA-histone subunit organization despite overall structural changes.