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Lactobacillus species associated with active caries lesions
S J Botha1, S C Boy, F S Botha
1Centre for Stomatological Research, University of Pretoria, South Africa.
The Journal of the Dental Association of South Africa = Die Tydskrif Van Die Tandheelkundige Vereniging Van Suid-Afrika
|May 20, 1998
Summary
Lactobacillus paracasei and Lactobacillus rhamnosus were the most common Lactobacillus species found in active dentine caries lesions. This study identified oral lactobacilli species composition in caries-active and caries-free individuals.
Area of Science:
- Microbiology
- Dental Research
- Oral Health
Background:
- Lactobacilli are implicated in the progression of dentine caries.
- Understanding the specific species involved is crucial for caries management.
Purpose of the Study:
- To isolate and identify Lactobacillus species associated with active dentine caries.
- To compare oral lactobacilli composition in caries-active versus caries-free individuals.
Main Methods:
- Samples from carious dentine (Group A) and stimulated saliva (Group B) were collected.
- Isolates were cultured on Rogosa agar, incubated anaerobically, and identified using classical taxonomy.
- 153 isolates were analyzed to determine species composition.
Main Results:
- Homofermentative species predominated in both groups (Group A: 82%, Group B: 90%).
- Lactobacillus paracasei and Lactobacillus rhamnosus were the most prevalent homofermentative species.
- Lactobacillus fermentum was the predominant heterofermentative species, especially in Group B (100%).
Conclusions:
- The study identified a distinct species composition of oral lactobacilli.
- Lactobacillus paracasei and Lactobacillus rhamnosus are key species in active dentine caries.
- Findings contribute to understanding the role of lactobacilli in the carious process.