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Determination of p-hydroxyphenylglycine by reaction with o-phthalaldehyde using a flow-injection fluorimetric
P Viñas1, N Campillo, C López-Erroz
1Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Spain.
Abstract:
In the present study, new flow injection procedures for the determination of p-hydroxyphenylglycine using either photometric or fluorimetric detection are proposed. The methods are based on the reaction of the amino acid with o-phthalaldehyde and 2-mercaptoethanol. The calibration graphs based on peak area were linear in the ranges 20-300 ng ml-1 with fluorimetric detection and 5-60 micrograms ml-1 using the photometric mode. The detection limit calculated for the fluorimetric procedure was 10 ng ml-1. The method was applied to the determination of the free p-hydroxyphenylglycine present in industrial pharmaceutical samples of a Dane salt.