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Related Experiment Videos

Sequence polymorphism in the bovine major histocompatibility complex DQB loci

L Nasir1, M Ndiaye, C Seely

  • 1Department of Veterinary Clinical Studies, University of Glasgow Veterinary School.

Animal Genetics
|May 20, 1998
PubMed
Summary

Investigating bovine DQB gene polymorphism in British Friesian cattle revealed novel sequences. High conservation in one DQB sequence suggests a low polymorphism locus, potentially similar to humans.

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Area of Science:

  • Immunogenetics
  • Veterinary Science
  • Molecular Biology

Background:

  • The bovine major histocompatibility complex (MHC) plays a crucial role in immune responses.
  • Polymorphism within MHC genes, particularly DQB, is vital for understanding cattle disease resistance and breeding.
  • Previous studies suggested limited DQB polymorphism in cattle.

Purpose of the Study:

  • To investigate the extent of polymorphism in the DQB sequences of the bovine MHC in British Friesian cattle.
  • To identify novel DQB sequences and compare them with known bovine and human DQB sequences.
  • To assess the conservation and potential pseudogene formation within the bovine DQB locus.

Main Methods:

  • Amplification of the first domain exon of bovine DQB sequences.

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  • Cloning and sequencing of the amplified DQB exon.
  • Bioinformatic analysis to identify sequence variations and conservation.

    • Main Results:

    • Eight distinct DQB sequences were identified in 22 British Friesian cattle.
    • Six of these sequences were novel, indicating undiscovered polymorphisms.
    • One DQB sequence was highly conserved, present in 21 out of 22 animals, and found across different cattle breeds.
    • A pseudogene with frameshift insertions was identified.

    Conclusions:

    • The bovine DQB locus exhibits a higher degree of polymorphism than previously assumed, with numerous novel sequences identified in British Friesians.
    • The high conservation of a specific DQB sequence suggests a functionally important, low-polymorphism locus, potentially analogous to the human DQB region.
    • The identification of a DQB pseudogene provides further insight into the evolutionary dynamics of the bovine MHC.