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Related Experiment Videos

Growth hormone treatment prevents the decrease in insulin-like growth factor I gene expression in patients undergoing

R Bjarnason1, R Wickelgren, M Hermansson

  • 1Department of Medicine, International Pediatric Growth Research Center, Goteborg, Sweden. ragnar.bjarnason@pediat.gu.se

The Journal of Clinical Endocrinology and Metabolism
|May 20, 1998
PubMed
Summary

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Growth hormone (GH) treatment prevents reduced insulin-like growth factor I (IGF-I) gene expression in skeletal muscle following major surgery. This finding is crucial for understanding and managing protein catabolism in surgical patients.

Area of Science:

  • Molecular Biology
  • Endocrinology
  • Surgical Metabolism

Background:

  • Major surgery, sepsis, and trauma increase protein catabolism, leading to acquired growth hormone (GH) resistance and reduced skeletal muscle mass.
  • GH treatment and glutamine-containing parenteral nutrition can mitigate this catabolism.
  • GH effects are partly mediated by insulin-like growth factor I (IGF-I), produced in the liver and target tissues.

Purpose of the Study:

  • To investigate the impact of GH administration on IGF-I and GH receptor (GHR) gene expression in skeletal muscle after major abdominal surgery.
  • To establish a quantitative RT-PCR assay for measuring IGF-I gene expression in muscle tissue.

Main Methods:

  • A quantitative RT-PCR assay was developed to measure IGF-I and GHR messenger RNA (mRNA) levels.

Related Experiment Videos

  • Skeletal muscle biopsies were obtained from patients undergoing elective abdominal surgery preoperatively and on postoperative day 3.
  • Patients received either GH plus glutamine-enriched total parenteral nutrition (TPN) or glutamine-enriched TPN alone (control).

    • Main Results:

    • In the control group, IGF-I mRNA levels significantly decreased from preoperative levels to postoperative day 3 (P < 0.05).
    • GH-treated patients showed no significant change in IGF-I mRNA levels between preoperative and postoperative samples.
    • No statistically significant changes in GHR gene expression were observed in either group.

    Conclusions:

    • GH administration effectively prevents the decline in IGF-I gene expression observed in skeletal muscle after major abdominal surgery.
    • This suggests a mechanism by which GH may counteract surgical-induced muscle catabolism.