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T and B cell development in BP-1/6C3/aminopeptidase A-deficient mice
Q Lin1, I Taniuchi, D Kitamura
1Department of Medicine, University of Alabama at Birmingham, 35294-3300, USA.
Journal of Immunology (Baltimore, Md. : 1950)
|May 20, 1998
Summary
BP-1 ectoenzyme activity is not essential for normal B and T cell development. Mice lacking BP-1 protein and enzyme activity showed no impairment in immune cell development or function, indicating its non-critical role in the immune system.
Area of Science:
- Immunology
- Molecular Biology
- Biochemistry
Background:
- Cell surface molecules delineate B cell differentiation in bone marrow.
- BP-1/6C3 antigen, also known as aminopeptidase A (APA), is expressed on pre-B and immature B cells.
- APA cleaves N-terminal acidic residues from peptides, modulating their activity.
Purpose of the Study:
- To investigate the physiological role of BP-1 ectoenzyme activity in B and T cell development.
- To determine if BP-1 deficiency impacts immune cell generation and function.
Main Methods:
- Gene targeting in embryonal stem cells to create BP-1 deficient mice.
- Phenotypic analysis of lymphocytes from bone marrow and thymus.
- Assessment of antibody responses to thymus-dependent and -independent antigens.
- Evaluation of B lymphopoiesis in fetal liver cultures and cell proliferation responses to IL-7 and LPS.
Main Results:
- BP-1 deficient mice showed normal development and produced normal numbers of T and B cells.
- Antibody responses and serum immunoglobulin levels were unaffected in BP-1 deficient mice.
- B and T cell differentiation, B lymphopoiesis, and cellular proliferation responses were unimpaired.
Conclusions:
- BP-1 ectoenzyme activity is not essential for normal B and T cell development and function.
- The absence of BP-1 does not compromise the integrity of the adaptive immune system in mice.