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Related Experiment Videos

The structure of avian CD5 implies a conserved function

R Koskinen1, T W Göbel, C A Tregaskes

  • 1Turku Immunology Centre and Department of Medical Microbiology, Turku University, Finland.

Journal of Immunology (Baltimore, Md. : 1950)
|May 20, 1998
PubMed
Summary

Chicken CD5, a crucial immune molecule, shares structural similarities with mouse and human counterparts. Its conserved signaling motifs suggest a vital role in T-cell development and immune function.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Genomics

Background:

  • CD5 is a transmembrane glycoprotein expressed on T lymphocytes.
  • Its precise function varies across species, necessitating further investigation in avian models.

Purpose of the Study:

  • To isolate and characterize the chicken CD5 cDNA.
  • To analyze the structure and expression pattern of chicken CD5.
  • To infer the functional role of chicken CD5 in avian immunity.

Main Methods:

  • COS cell expression cloning using a novel monoclonal antibody (mAb 2-191).
  • Nucleotide sequencing and protein homology analysis.
  • Immunoprecipitation and SDS-PAGE to determine protein characteristics.
  • Flow cytometry or similar techniques to analyze CD5 expression on different immune cells.

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Main Results:

  • Chicken CD5 cDNA was successfully isolated, encoding a protein with significant homology to mouse and human CD5.
  • The deduced protein structure includes scavenger receptor cysteine-rich domains, a transmembrane domain, and a conserved cytoplasmic tail with phosphorylation sites.
  • Chicken CD5 is a 64-kDa phosphorylated glycoprotein.
  • CD5 is highly expressed on chicken alpha-beta T cells, with variable expression on gamma-delta T cells and low expression on B cells.

Conclusions:

  • Chicken CD5 shares key structural and functional motifs with mammalian CD5, indicating conserved roles in lymphocyte development and signaling.
  • The distinct expression patterns suggest species-specific regulation and functions of CD5 in the avian immune system.