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Related Experiment Videos

Regionalization defects in the weaver mouse cerebellum

L M Eisenman1, E Gallagher, R Hawkes

  • 1Department of Pathology, Anatomy, and Cell Biology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107-6799, USA.

The Journal of Comparative Neurology
|May 20, 1998
PubMed
Summary

The weaver (wv) mutation disrupts cerebellar development in mice, altering zebrin II expression patterns and Purkinje cell distribution. This highlights the mutation's potential for understanding cerebellar regionalization.

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Genetics

Background:

  • The mammalian cerebellum exhibits distinct parasagittal bands and transverse zones established during development.
  • Purkinje cell compartmentation, visualized by zebrin II (aldolase C) immunostaining, reflects these developmental patterns.
  • Zebrin II expression is cell-autonomous, suggesting pattern disruptions indicate early developmental issues.

Purpose of the Study:

  • To investigate the effects of the weaver (wv) mutation on cerebellar development and zebrin II expression patterns in mice.
  • To determine if the wv mutation alters Purkinje cell compartmentation and its underlying mechanisms.

Main Methods:

  • Immunostaining of adult weaver (wv/wv) mouse cerebella for zebrin II.
  • Analysis of zebrin II expression patterns (zebrin II+ and zebrin II- Purkinje cells) in different cerebellar regions.

Related Experiment Videos

  • Serial reconstruction to examine cytoarchitectonic defects and identify transverse boundaries.

    • Main Results:

    • Homozygous weaver (wv/wv) mice display abnormal distribution of zebrin II+ and zebrin II- Purkinje cells.
    • Posterior vermis shows wider, multilaminate zebrin II+ bands, while central vermis lacks a zebrin II+ cell mass, and anterior lobe shows missing zebrin II- bands.
    • Cytoarchitectonic defects are linked to two transverse boundaries in lobules VI and IX, suggesting a deleted transverse zone may underlie abnormal zebrin expression.

    Conclusions:

    • The weaver (wv) mutation significantly alters Purkinje cell compartmentation, a novel finding.
    • Abnormal zebrin II expression in wv/wv mice is likely secondary to the deletion of a cerebellar transverse zone.
    • The wv mutation provides a valuable tool for studying cerebellar regionalization and pattern formation.