[Changes of Kupffer cell DNA and RNA synthetic functions after partial hepatectomy in cirrhotic rats]

We studied the changes of DNA and RNA synthetic functions of regenerating liver cells after partial hepatectomy in cirrhotic rats and discussed the relationship between liver regeneration and liver functional failure. Seventy healthy male Wistar rats weighing 200 approximately 250 g, were randomized into sham-operated (SO), partial hepatectomy in cirrhotic rats (C-PH) and partial hepatectomy in normal rats (N-PH). The left and middle lobes were dissected. The synthetic DNA peak of Kupffer cells (KC) in the G2 and M phase in C-PH was found after 24 hr of operation. The regenerating index peak of KC was at 48 hr after operation, which was higher than the other groups, but the liver weights in C-PH was lower than that in the N-PH at 1 week after operation. The RNA content of KC markedly decreased and returned to normal levels at 1 week after operation. We think that KC functions are suppressed after partial hepatectomy in cirrhotic rats. It is an important cause of hepatocyte regeneration function decrease and liver functional failure for delay of liver cell regeneration "start" and the regeneration cell cycle.