[An experimental study on the anticancerous effects of CD3-TIL derived from human primary hepatic carcinoma]

In this study, CD3-TIL produced by in vito induction with CD3 and IL-2 (CD3-TIL) was compared with TIL stimulated by only IL-2 (TIL) in their in vitro proliferation, and both in vitro and in vivo anticancerous effects. It was found that CD3 at the concentration of 100 ng/ml was the most suitable stimulus of in vitro TIL proliferation (CD3-TIL) which was significantly greater than that by simple IL-2 stimulation. Also in vitro anticancerous activity of CD3-TIL was greater than TIL. CD3-TIL significantly inhibited tumor growth, causing shrinkage of tumor mass, and prolonging the life span of the nude mouse with tumor burden.