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Detection of ABH blood group antigens from minute human hairs using polyvinylidene difluoride membrane with
H Biwasaka1, N Nakayashiki, Y Aoki
1Department of Legal Medicine, Iwate Medical University School of Medicine, Morioka, Japan.
Nihon Hoigaku Zasshi = the Japanese Journal of Legal Medicine
|May 20, 1998
Abstract:
A sensitive and simple method for ABH blood grouping of human hairs using a polyvinylidene difluoride (PVDF) membrane was described. The hairs sensitized with monoclonal antibodies were applied onto the membrane and heated at 60 degrees C on a hot plate. When the membrane was examined with ELISA using a peroxidase-conjugated anti-IgM antibody, group-specific streaky stains were perceived on the membrane, because the eluates directly bound to the membrane. The present method could be available to the detection of minute antigens of short hairs.