Related Experiment Video

Updated: Aug 17, 2026

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
10:51

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists

Published on: November 15, 2013

Identification of domains of c-Jun mediating androgen receptor transactivation

S C Wise1, L A Burmeister, X F Zhou

  • 1University of Toledo, Department of Biology, Ohio 43606, USA.

Oncogene
|May 20, 1998
PubMed

Insights

The proto-oncoprotein c-Jun indirectly stimulates androgen receptor transactivation via its activation functions, independent of its DNA-binding ability. This study identifies key c-Jun domains essential for this distinct regulatory mechanism.

Area of Science:

  • Molecular Biology
  • Oncogenesis
  • Gene Regulation

Background:

  • The proto-oncoprotein c-Jun, as part of the AP-1 complex, regulates gene transcription via DNA binding.
  • Previously, an indirect mechanism of c-Jun regulating transcription by stimulating androgen receptor (AR) transactivation was reported, independent of c-Fos or DNA binding.

Purpose of the Study:

  • To characterize the specific domains of c-Jun responsible for stimulating androgen receptor transactivation.
  • To investigate the role of c-Jun's transactivation ability in this indirect mechanism.

Main Methods:

  • Utilized a series of c-Jun mutants to identify critical domains.
  • Employed c-Jun/v-Jun chimeras to assess functional differences.
  • Analyzed c-Jun's effect on androgen receptor-mediated transactivation using a transactivation-deficient mutant.

Main Results:

  • A functional basic leucine zipper (bZIP) region and N-terminal activation functions of c-Jun are necessary for stimulating AR transactivation.
  • v-Jun cannot stimulate AR transactivation, highlighting the importance of c-Jun's specific activation functions.
  • c-Jun exhibits bell-shaped activity on AR transactivation, distinct from its own transactivation capacity.
  • A c-Jun mutant lacking transactivation ability can still stimulate AR activity.

Conclusions:

  • c-Jun's ability to stimulate androgen receptor transactivation is separable from its intrinsic transactivation function.
  • The N-terminal activation functions of c-Jun are crucial for its indirect regulation of AR.
  • This finding reveals a novel, DNA-binding-independent regulatory role for c-Jun in androgen receptor signaling.

More Related Videos

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation09:07

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Published on: June 21, 2016

Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay09:07

Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay

Published on: December 19, 2018

Related Concept Videos

Internal Receptors01:31

Internal Receptors

Many cellular signals are hydrophilic and therefore cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind to internal, or intracellular, receptors that reside within the cell. Many mammalian steroid hormones use this mechanism of cell signaling, as does nitric oxide (NO) gas.
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Signal Transduction: Overview01:26

Signal Transduction: Overview

Cells respond to many types of information, often through receptor proteins positioned on the membrane. They respond to chemical signals, such as hormones, neurotransmitters, and other signaling molecules, initiating a series of molecular reactions to produce an appropriate response. This is called signal transduction. Cells also coordinate different responses elicited by the same signaling molecule via mediators, allowing molecular cross-talk.
Typically, signal transduction involves three...