Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sticky egyptians: a technique for assembling genes encoding constrained peptides of variable length

S J Palmer1, M R Redfern, G C Smith

  • 1Department of Chemistry and Department of Mathematical Sciences, University of Bath, Bath BA2 7AY, UK.

Nucleic Acids Research
|May 21, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Influence of Certain Processing Steps on Attachment of Microorganisms to Pork Skin <sup>1</sup>.

Journal of food protection·2019
Same author

Bacteriology of Electrically Stimulated and Unstimulated Rabbit, Pork, Lamb and Beef Carcasses.

Journal of food protection·2019
Same author

Effect of CO<sub>2</sub>-N<sub>2</sub> Atmospheres on the Microbial Flora of Pork.

Journal of food protection·2019
Same author

Physical and Sensory Characteristics of Pork Loins Stored in Vacuum or Modified Atmosphere Packages.

Journal of food protection·2019
Same author

Role of Hafnia alvei and a Lactobacillus Species in the Spoilage of Vacuum-Packaged Strip Loin Steaks.

Journal of food protection·2019
Same author

Blade Tenderization Effects on Cooking and Palatability Characteristics of Steaks from Bullock and Cow Carcasses.

Journal of food protection·2019

Researchers developed a new method to create diverse peptide shapes for therapeutic and diagnostic applications. This technique assembles DNA fragments encoding peptides with an even number of cysteine residues and variable lengths, enabling a peptide shape library.

Area of Science:

  • Biochemistry and Molecular Biology
  • Peptide Chemistry
  • Drug Discovery and Development

Background:

  • Naturally occurring peptides, particularly from Conus snails, exhibit high specificity in molecular interactions.
  • Peptide structure, rigidity, and shape are primarily determined by disulfide bonds, peptide length, and cysteine residue distribution.
  • Complementary shapes are crucial for effective molecular interactions, suggesting therapeutic and diagnostic potential in shape-diverse peptide libraries.

Purpose of the Study:

  • To develop a method for constructing a peptide shape library.
  • To enable the creation of peptides with diverse and predictable molecular shapes.
  • To facilitate the identification of peptides with shapes complementary to specific biological targets for therapeutic or diagnostic use.

Main Methods:

Related Experiment Videos

  • A novel method for assembling DNA fragments was developed.
  • The method focuses on creating DNA sequences that encode peptides with an even number of cysteine residues.
  • The DNA fragments are of variable lengths, allowing for a range of peptide conformations and shapes.

Main Results:

  • A method for assembling DNA fragments encoding peptides with an even number of cysteine residues and variable lengths has been successfully developed.
  • This method represents a foundational step towards building a comprehensive library of peptide shapes.
  • The described technique allows for the controlled generation of diverse peptide structures.

Conclusions:

  • The developed DNA assembly method is a key advancement for creating peptide shape libraries.
  • This approach holds significant promise for identifying novel therapeutic and diagnostic agents.
  • Further research can leverage this method to explore the vast potential of shape-based peptide discovery.