Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Combinational variation of restriction modification specificities in Lactococcus lactis

C Schouler1, M Gautier, S D Ehrlich

  • 1INRA, Laboratoire de Génétique Microbienne, Jouy-en-Josas, France.

Molecular Microbiology
|May 21, 1998
PubMed
Summary

Lactococcus lactis possesses chromosomal genes for restriction-modification (R-M) systems. Plasmids carrying specific subunits allow for novel R-M combinations, enhancing bacterial defense against foreign DNA.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic and Morphological Diversity in Spontaneous Populations of Brassica rapa: How Do Feral Populations Differ From Wild Ones?

Molecular ecology·2026
Same author

Revue des maladies respiratoires·2025
Same author

Corrigendum to "Update of guidelines for management of community acquired pneumonia in adults by the French infectious disease society (SPILF) and the French-speaking society of respiratory diseases (SPLF). Endorsed by the French intensive care society (SRLF), the French microbiology society (SFM), the French radiology society (SFR) and the French emergency society (SFMU)" [IDNOW 55/2 (2025) 105034].

Infectious diseases now·2025
Same author

[Update of guidelines for management of Community Acquired pneumonia in adults by French Infectious Disease Society (SPILF) and the French Speaking Society of Respiratory Diseases (SPLF). Endorsed by French intensive care society (SRLF), French microbiology society (SFM), French radiology society (SFR), French emergency society (SFMU)].

Revue des maladies respiratoires·2025
Same author

The inverse square law: A basic principle in brachytherapy.

Cancer radiotherapie : journal de la Societe francaise de radiotherapie oncologique·2022
Same author

Brachytherapy: An emblematic example of extreme hypofractionated regimen.

Cancer radiotherapie : journal de la Societe francaise de radiotherapie oncologique·2021

Area of Science:

  • Molecular Biology
  • Bacteriology
  • Genetics

Background:

  • Restriction-modification (R-M) systems are crucial for bacterial defense against foreign DNA.
  • Type I R-M systems, involving HsdR, HsdM, and HsdS subunits, exhibit diverse specificities.
  • Lactococcus lactis is a significant bacterium in food fermentation and probiotic applications.

Purpose of the Study:

  • To identify and characterize type I R-M systems in Lactococcus lactis strain IL1403.
  • To investigate the role of plasmid-encoded subunits in R-M system variation.
  • To understand the implications of R-M system plasticity for bacterial adaptation.

Main Methods:

  • Genomic and plasmid DNA analysis of Lactococcus lactis IL1403.
  • Gene identification and sequencing of R-M system components.

Related Experiment Videos

  • Phenotypic analysis of R-M activity in modified bacterial strains.

    • Main Results:

    • Three chromosomal genes encoding type I R-M system components (related to class C enzymes) were identified in L. lactis IL1403.
    • Plasmids encoding only the HsdS subunit, which determines R-M specificity, were discovered.
    • The presence of these plasmids in IL1403 resulted in a new R-M phenotype, demonstrating functional interaction between plasmid- and chromosome-encoded subunits.

    Conclusions:

    • Combinatorial variation of type I R-M systems through plasmid-chromosome interactions is a mechanism for evolving R-M specificity.
    • This adaptability enhances bacterial resistance to invasive foreign DNA.
    • The findings provide insights into the evolutionary dynamics of bacterial defense systems.