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Treatment of equine oocytes with A23187 after intracytoplasmic sperm injection
H Kato1, G E Seidel, E L Squires
1Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins 80523, USA.
Equine Veterinary Journal. Supplement
|May 21, 1998
Abstract:
In vitro matured horse oocytes with a first polar body (n = 68) were each injected with a single spermatozoon and divided into 2 groups: Group 1 oocytes were treated with 10 microM calcium ionophore A23187 for 5 min while Group 2 oocytes received no activation treatment. After culture in vitro for 2 days, significantly more oocytes treated with A23187 (5/24, 21%) cleaved than oocytes without activation treatment (2/44, 5%, P<0.05). All 7 cleaved zygotes from both treatment groups were transferred to recipient mares but no pregnancies resulted.