Chloride-sulphate exchange chemically measured in human erythrocyte ghosts

The purpose of this paper is to provide a clear description of a new chemical method to measure and analyze the net anion exchange (Cl-/SO4--) in resealed human erythrocyte ghosts. With this technique, we measured the influx of an inorganic divalent anion (SO4--) against a monovalent anion (Cl-) as a function of temperature. We found an apparent activation enthalpy Ea of 21-24 Kcal/mol. Besides the Cl-/SO4-- influx pH - dependence, measured under condition of electric anion equilibrium, shows a bell shape with a maximum around pH 6.3. In addition we accounted for the extracellular pH changes during the exchange of chloride for sulphate across the erythrocyte ghosts when bicarbonate is present in the medium. The pH drops sharply, then increases for the remaining experimental time course.