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Decrease in nucleoside diphosphate kinase (NDPK/nm23) expression during hematopoietic maturation

R Willems1, D R Van Bockstaele, F Lardon

  • 1Laboratory of Experimental Hematology, Department of Medicine, University of Antwerp, Antwerp University Hospital (UIA/UZA), Wilrijkstraat 10, B-2650 Edegem, Belgium.

The Journal of Biological Chemistry
|June 5, 1998
PubMed
Summary
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Nucleoside diphosphate kinase (NDPK/nm23) is crucial in early hematopoiesis, with levels decreasing during cell differentiation. Its expression increases in activated lymphocytes, suggesting a role in immune responses.

Area of Science:

  • Hematology
  • Molecular Biology
  • Cell Biology

Background:

  • Nucleoside diphosphate kinase (NDPK), also known as nm23, comprises several isoforms, including H1 and H2.
  • NDPK plays roles in various cellular processes, including nucleotide metabolism and signal transduction.

Purpose of the Study:

  • To investigate the expression and localization of NDPK isoforms H1 and H2 in hematopoietic tissues.
  • To determine the role of NDPK in hematopoiesis and immune cell activation.

Main Methods:

  • Flow cytometry was employed to analyze intracellular and extracellular NDPK concentrations.
  • Enzymatic assays were performed to quantify NDPK activity.
  • Multiparameter flow cytometry was used to assess cell cycle-dependent expression changes.

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Main Results:

  • Highest intracellular levels of nm23-H1 and nm23-H2 were found in bone marrow CD34(+) progenitors.
  • NDPK expression decreased in more mature bone marrow cells and peripheral blood leukocytes.
  • nm23 expression was upregulated in lymphocytes upon phytohemagglutinin-induced proliferation, specifically during the G0/G1-transition.
  • Weak surface expression of nm23 was detected on some hematopoietic cell lines but not on normal hematopoietic cells.
  • NDPK was found in human plasma, likely due to red blood cell lysis.

Conclusions:

  • NDPK/nm23 isoforms are important in early hematopoiesis and are downregulated during cell differentiation.
  • NDPK expression is upregulated in activated lymphocytes, indicating a potential role in immune responses.
  • The presence of NDPK in plasma may be linked to red blood cell turnover.