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Related Experiment Videos

Neurally mediated hyperactive voiding in spontaneously hypertensive rats

J M Spitsbergen1, D B Clemow, R McCarty

  • 1Department of Neuroscience, Box 230, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA.

Brain Research
|June 17, 1998
PubMed
Summary
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Spontaneously hypertensive rats exhibit increased nerve growth factor (NGF) secretion by bladder smooth muscle, leading to hyperinnervation and hyperactive voiding. This suggests a genetic model for studying bladder dysfunction.

Area of Science:

  • Urology
  • Physiology
  • Neuroscience

Background:

  • Hypertension and bladder overactivity share mechanisms like abnormal smooth muscle growth and altered nerve growth factor (NGF) levels.
  • Spontaneously hypertensive rats (SHR) and obstructed rats show similar pathological changes in bladder smooth muscle and innervation.

Purpose of the Study:

  • To investigate if bladder smooth muscle in SHR secretes elevated NGF.
  • To determine if increased NGF contributes to altered bladder innervation and function without hypertrophy in SHR.
  • To explore the SHR strain as a model for studying bladder dysfunction related to innervation changes.

Main Methods:

  • Voiding behavior was monitored using metabolic cages.
  • NGF levels were quantified using ELISA in tissue and cell culture.

Related Experiment Videos

  • NGF mRNA was measured via reverse transcriptase PCR in bladder smooth muscle cells (BSMCs).
  • Noradrenergic innervation was assessed through staining and norepinephrine (NE) content analysis via HPLC.

    • Main Results:

    • SHRs demonstrated more frequent voiding compared to Wistar-Kyoto (WKY) rats.
    • Adult SHR bladders had higher NGF content than WKY bladders.
    • SHR BSMCs secreted NGF at a higher rate per unit mRNA than WKY BSMCs, despite similar mRNA levels.
    • SHR bladders exhibited greater NE content and denser catecholaminergic innervation.

    Conclusions:

    • Elevated NGF secretion by bladder smooth muscle is linked to bladder hyperinnervation and hyperactive voiding in SHR.
    • The SHR model is suitable for investigating bladder dysfunction arising from altered innervation patterns.