Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Actin Filament Depolymerization

Actin Filament Depolymerization

Actin filaments (F-actin) are composed of actin subunits. The dissociation of actin monomers can occur from either end of F-actin. The rate of dissociation is faster from the minus-end or the pointed end, where the actin subunits exist with a bound ADP, together known as ADP-actin. The depolymerization of F-actin is aided by proteins, including the actin-depolymerizing factor (ADF) and cofilin family of proteins, gelsolin, and glia maturation factor (GMF).
In F-actin, the ADF/cofilin proteins...

Mechanism of Filopodia Formation

Mechanism of Filopodia Formation

Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Human sperm motility, capacitation and acrosome reaction are impaired by 2-arachidonoylglycerol endocannabinoid.

Histology and histopathology·2017

Same author

Capacitation and acrosome reaction changes α-tubulin immunodistribution in human spermatozoa.

Reproductive biomedicine online·2013

Same author

Mitochondrial DNA variability of the pipefish Syngnathus abaster.

Journal of fish biology·2013

Same author

Characterization of the lectin binding pattern in human spermatozoa after swim-up selection.

Histology and histopathology·2012

Same author

[Reasons for not initating HCV treatment in prison: a subanalysis of the EPIBAND study].

Revista espanola de sanidad penitenciaria·2011

Same author

The Bronze Age burials from Cova Dels Blaus (Vall d'Uixó, Castelló, Spain): an approach to palaeodietary reconstruction through dental pathology, occlusal wear and buccal microwear patterns.

Homo : internationale Zeitschrift fur die vergleichende Forschung am Menschen·2007

Search research articles

Related Experiment Video

Updated: Jul 17, 2026

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

Published on: April 10, 2016

Interocular effect of actin depolymerization on spinule formation in teleost retina

J De Juan¹, M García

¹Departamento de Biotecnología, Facultad de Ciencias, Universidad de Alicante, Apdo. Correos 99, Alicante 03080, Spain.

|June 24, 1998

Summary

Actin filaments are crucial for spinule formation in teleost retinas. Disrupting actin with cytochalasin D reduces spinules and alters horizontal cell nematosomes, impacting light adaptation.

More Related Videos

Assessment of Zebrafish Lens Nucleus Localization and Sutural Integrity

Assessment of Zebrafish Lens Nucleus Localization and Sutural Integrity

Published on: May 6, 2019

Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System

Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System

Published on: February 11, 2022

Related Experiment Videos

Last Updated: Jul 17, 2026

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

Published on: April 10, 2016

Assessment of Zebrafish Lens Nucleus Localization and Sutural Integrity

Assessment of Zebrafish Lens Nucleus Localization and Sutural Integrity

Published on: May 6, 2019

Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System

Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System

Published on: February 11, 2022

Area of Science:

Ophthalmology
Cell Biology
Neuroscience

Background:

Teleost retinas exhibit light-dependent structural changes, including spinule formation in cone pedicles.
Horizontal cells in the retina possess nematosomes, structures whose size inversely correlates with spinule number.

Purpose of the Study:

To investigate the role of actin filaments in the formation of retinal spinules.
To understand the relationship between actin, spinules, and nematosomes in teleost retinas.

Main Methods:

Intraocular injection of cytochalasin D, an actin inhibitor, into one eye of a teleost.
Ultrastructural analysis of retinal tissues from both treated and contralateral untreated eyes.

Main Results:

Cytochalasin D treatment significantly impaired the formation of spinules in cone pedicles.
The size reduction of nematosomes in horizontal cells was also inhibited by cytochalasin D.
These effects were observed in both the treated and the untreated contralateral retinas, suggesting a systemic effect.

Conclusions:

Actin filaments play a critical role in the dynamic regulation of spinule formation in teleost retinas.
The interplay between actin, spinules, and nematosomes is essential for retinal light adaptation mechanisms.
Cytochalasin D's impact on both treated and untreated retinas highlights the complex signaling pathways involved.