[Immunological comparison of differently treated allografts of bone]

The immunnologic rejection induced by differently treated allografts of bone was compared. Methods Fresh bone (FB), autolyzed antigen-free bone (AAA), bone matrix gelatin (BMG), demineralized bone matrix (DBM) were implanted into the muscle pouch of mice, then, the immunological tests and alkaline phosphatase assay were conducted. Results Allogeneic FB induced the highest level of serum antibody in the host and stimulated lymphocytes proliferation remarkably in vitro; in contrast, AAA, BMG and DBM caused low titer of antibody and inhibited lymphocytes reproduction in vitro. Conclusions. Immunological rejection restrained osteogenesis of the bone implant, whereas the osteoinductive substance of bone suppressed immunological reaction.