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Expression of proliferating cell nuclear antigen in Wilms' tumor
1Department of Pediatric Surgery, Xin Hua Hospital, Shanghai Second Medical University, China.
Objective:
To investigate the expression of proliferating cell nuclear antigen (PCNA) and its clinical significance in Wilms' tumor.
Methods:
PCNA immunoreactivity on paraffin-embedded tissues was assessed retrospectively in 35 cases using labelled streptaridin biotin (LSAB) procedure, and its association with clinical and other biological features was studied.
Results:
Twelve tumors (34.29%) expressed PCNA. Expression rate increased with the advance of stage (P < 0.05). The S-phase fraction and proliferation index (PI) in children with PCNA positive expression were higher than those in children with negative PCNA expression (P < 0.05). The survival rate (16.67%) in patients with positive PCNA expression was significantly lower than that (73.91%) in no-expression ones (P < 0.01).
Conclusion:
PCNA is a sensitive indicator for cell proliferation and is clinically useful in identifying a poor prognostic group of patients with Wilms' tumor.