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The three-for-one model for gram-negative wall growth: a problem and a possible solution

A L Koch1

  • 1Biology Department, Indiana University, Bloomington 47405, USA. Koch@indiana.edu

FEMS Microbiology Letters
|May 22, 1998
PubMed
Summary

Gram-negative bacteria grow via a complex murein wall enlargement model. Turgor pressure may stretch wall components, allowing the

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Area of Science:

  • Microbiology
  • Biophysics

Background:

  • Gram-negative bacteria possess a thin murein wall, necessitating complex growth mechanisms.
  • Höltje's 'three-for-one' model proposes murein wall growth through trimer formation and autolysis.
  • The model faces challenges due to precise fitting requirements between rafts and the stress-bearing wall.

Purpose of the Study:

  • To investigate the feasibility of the 'three-for-one' murein wall growth model.
  • To explore the role of cell turgor pressure in compensating for structural constraints.

Main Methods:

  • Analysis of murein components.
  • Theoretical modeling of wall growth.
  • Calculation of component stretching under turgor pressure.

Main Results:

  • Cell turgor pressure can stretch glycan chains by 10% and peptide strands by 28%.
  • This stretching compensates for the 'honeycomb' structure of the stressed wall.
  • Stretching allows for the precise fitting required by the 'three-for-one' model.

Conclusions:

  • Cell turgor pressure is crucial for enabling stable and indefinite murein wall enlargement.
  • The proposed stretching mechanism reconciles the 'three-for-one' model with wall mechanics.

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