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Bacterial utilization of cyclo(glycl-L-prolyl)
Experientia
|May 15, 1978
Summary
Soil bacteria utilize cyclo(Gly-L-Pro) as a nutrient source, requiring yeast extract for optimal growth. This dipeptide is excreted intact by rats, unlike its linear form.
Area of Science:
- Microbiology
- Biochemistry
Background:
- Soil bacteria possess diverse metabolic capabilities.
- Dipeptides can serve as nutrient sources for microorganisms.
Purpose of the Study:
- To isolate and characterize soil bacteria capable of utilizing cyclo(Gly-L-Pro).
- To investigate the metabolic fate of cyclo(Gly-L-Pro) in a mammalian model.
Main Methods:
- Bacterial isolation using cyclo(Gly-L-Pro) as a sole carbon/nitrogen source.
- Growth studies with and without yeast extract supplementation.
- In vivo metabolic studies in albino rats using radiolabeled compounds.
Main Results:
- Three bacterial strains (two Achromobacter, one Flavobacterium) were isolated.
- Growth on cyclo(Gly-L-Pro) was inducible and required yeast extract.
- Injected cyclo(Gly-L-Pro) was excreted intact by rats, while H-Gly-L-Pro-OH was metabolized.
Conclusions:
- Soil bacteria can metabolize cyclic dipeptides.
- Cyclo(Gly-L-Pro) metabolism is inducible in these bacterial strains.
- Mammalian metabolism differs significantly between cyclic and linear dipeptides.