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[Comparison of local antigen-specific IgA responses with serum IgA response after intrapulmonary antigen immunization
Objective:
To observe local lung and serum antigen-specific IgA response after local lung antigen immunization (LLI).
Methods:
11 healthy subjects were immunized with keyhole limpet hemocyanine (KLH) through bronchoscopy. 10-14 days after immunization, IgA responses were obversed in bronchoalveolar lavage fluids (BALF) and serum.
Results:
LLI induced anti-KLH IgA response in immunized BALF and serum, and both were polymeric IgA. Anti-KLH IgA2/IgA1 ratios were greater in immunized BALF than in serum. Anti-KLH IgA activity in immunized BALF was associated with secretory component (SC), but in serum was not correlated with SC.
Conclusions:
LLI could induce local antigen-specific IgA production. There were markedly differences between the structure and subclass distribution of antigen-specific IgA in immunized BALF and serum.