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[The inhibitory effect of recombinant human C3 fragment on murine endotoxic shock]
Objective:
To study the potentially practical use of C3 inactive fragment in anti-inflammation.
Methods:
A vector expressing RGD polypeptide derived from the alpha chain segment of human C3 was constructed by using PCR and genetic engineering methods and a recombinant protein (namely C 33) was expressed with high efficiency in E. coli.
Results:
The analysis of SDS-PAGE showed the molecular weight of C 33 was about 15 KD. Its purity was above 95% after purification. The amino acid composition was inconsistent with the theoretical values. U937 cells stimulated by low dosage PMA adhered with coated C 33, and the adhesion was blocked by anti-CD 11b monoclonal antibody. After injection of purified C 33 into mice which were consequently challenged by dead E. coli, the mortality of the endotoxic shock was significantly reduced.
Conclusion:
C 33 can specifically bind to CD 11b/CD 18. C 33 as a ligand for CD 11b/CD 18 might be potentially used as an anti-inflammatory agent.