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[The preliminary study on prevention and treatment of neonatal jaundice]
Objective:
The purification and identification of heme oxygenase isoforms (HO-1) and HO-2 from human liver were described and Sn-protoporphyrin (SnPP) was used to inhibit HO-1 activity in order to provide a new method for prevention and treatment of neonatal jaundice.
Methods:
Human hepatic microsomal fractions were purified by DEAE-Sephadex A-25 and hydroxylapatite chromatography. The enzyme activities of the two isoforms were detected with and without Snpp.
Results:
HO-1 was the predominant form with a ratio of 2.4:1 (HO-1:HO-2). The apparent molecular weight of HO-1 and HO-2 on SDS-PAGE was 30,000 and 36,000 under reducing conditions, respectively. The study also showed that HO-1 was the traditional inducible heme oxygenase and HO-2 was a non-inducible heme oxygenase.
Conclusion:
The efficient inhibition effect of SnPP on HO-1 is suggested.