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Related Experiment Videos

Cellular localization and expression of template-activating factor I in different cell types

K Nagata1, S Saito, M Okuwaki

  • 1Department of Biomolecular Engineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.

Experimental Cell Research
|May 23, 1998
PubMed
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Template-activating factors I (TAF-I) are chromatin remodelers found across species. Their nuclear localization is crucial for function, though variations exist in specific cell types and during the cell cycle.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Template-activating factors I (TAF-I) alpha and beta are identified chromatin remodeling factors in human HeLa cells.
  • TAF-I beta is linked to the SET gene, implicated in acute undifferentiated leukemia through a SET-CAN fusion gene.

Purpose of the Study:

  • To determine the cellular localization of TAF-I proteins.
  • To investigate the presence and distribution of TAF-I homologs in various organisms and tissues.
  • To examine TAF-I expression during the cell cycle and the impact of its acidic region on localization.

Main Methods:

  • Generation of polyclonal and monoclonal antibodies against TAF-I.
  • Immunological detection of TAF-I in human, mouse, frog, insect, and yeast cells.

Related Experiment Videos

  • Analysis of TAF-I levels in synchronized HeLa and NIH 3T3 cells.
  • Comparison of localization for full-length TAF-I and TAF-I lacking the acidic region.

    • Main Results:

    • TAF-I proteins are conserved across diverse species, including mammals, amphibians, insects, and yeast.
    • Mouse TAF-I homolog is ubiquitously expressed in various tissue types.
    • TAF-I alpha and beta levels vary in hematopoietic cells, with some cell types lacking TAF-I alpha.
    • TAF-I protein levels remain stable during cell cycle progression.
    • Full-length TAF-I is predominantly nuclear, while TAF-I lacking the acidic region exhibits both nuclear and cytoplasmic localization.

    Conclusions:

    • TAF-I proteins are evolutionarily conserved chromatin remodeling factors with a primary nuclear localization essential for their activity.
    • Variations in TAF-I expression among cell types and the functional significance of its acidic region warrant further investigation into TAF-I's regulatory mechanisms.