Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Maize polyamine oxidase: primary structure from protein and cDNA sequencing

P Tavladoraki1, M E Schininà, F Cecconi

  • 1Dipartimento di Biologia, Università Roma Tre, Rome, Italy.

FEBS Letters
|May 23, 1998
PubMed
Summary

Researchers determined the first complete amino acid sequence of maize flavin-containing polyamine oxidase using combined nucleotide and peptide analysis. This study reveals the protein

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Calorie-restriction treatment mitigates the aging in rat liver model.

Biogerontology·2025
Same author

Association of peripheral monocytic myeloid-derived suppressor cells with molecular subtypes in single-center endometrial cancer patients receiving carboplatin + paclitaxel/avelumab (MITO-END3 trial).

Cancer immunology, immunotherapy : CII·2025
Same author

Minimally invasive management of chronic venous insufficiency: A case report on combined radiofrequency ablation and sclerotherapy in an obese patient.

International journal of surgery case reports·2025
Same author

Macroalgal blooms affect the food web of tropical coastal ecosystems impacted by fisheries.

Marine environmental research·2023
Same author

Influence of the river flow pulse on the maturity, resilience, and sustainability of tropical coastal ecosystems.

Marine environmental research·2022
Same author

DPO: Diffuse Pulmonary Ossification - A Diagnostic Challenge.

Translational medicine @ UniSa·2022

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Plant Science

Background:

  • Flavin-containing polyamine oxidases (FPAsO) are crucial enzymes involved in polyamine catabolism.
  • Understanding the primary structure of FPAsO is essential for elucidating their function and evolutionary relationships.

Purpose of the Study:

  • To determine the complete amino acid sequence of a flavin-containing polyamine oxidase from maize.
  • To confirm the primary structure through independent peptide sequencing.
  • To investigate structural relationships with related enzymes.

Main Methods:

  • Isolation and sequencing of a 1737 bp cDNA from maize seedlings using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE).
  • Analysis of the cDNA sequence to predict the polypeptide chain of 500 amino acids.

Related Experiment Videos

  • Extensive amino acid sequencing of the mature protein to confirm the primary structure.

    • Main Results:

    • The first complete amino acid sequence of a maize flavin-containing polyamine oxidase was successfully elucidated.
    • The deduced sequence indicated a polypeptide chain of 500 amino acids with a predicted secretion signal peptide.
    • Independent peptide sequencing confirmed the primary structure of the mature protein.

    Conclusions:

    • The complete amino acid sequence provides a foundation for understanding maize FPAsO structure-function relationships.
    • The findings offer insights into the evolutionary connections between flavin-containing polyamine oxidases and flavin-containing monoamine oxidases.