Related Experiment Video
Updated: Aug 9, 2026

Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography
Published on: September 14, 2014
Very-long-chain acyl-CoA dehydrogenase subunit assembles to the dimer form on mitochondrial inner membrane
M Souri1, T Aoyama, G Hoganson
1Department of Biochemistry, Shinshu University School of Medicine, Matsumoto, Nagano, Japan. msouri@med.id.yamagata-u.ac.jp
Abstract:
This paper describes the process of dimer assembly of mitochondrial very-long-chain acyl-CoA dehydrogenase (VLCAD) subunit. Mature VLCAD is a homodimer of a 70-kDa protein associated with the mitochondrial membrane. Newly synthesized VLCAD was present as a monomer and the major fraction was associated with the mitochondrial inner membrane. The association of VLCAD subunit with the mitochondrial membrane was observed early during dimer formation. In contrast, a VLCAD monomeric mutant S583W, a novel mutation identified from a patient with VLCAD deficiency, did not associate with the mitochondrial membrane after import and the major fraction remained in the mitochondrial matrix. These results suggest that association of VLCAD protein with mitochondrial inner membrane is necessary for dimer assembly and formation of mature VLCAD.
More Related Videos
Related Concept Videos
Electron Transport Chains
The ETC is comprised of...
The Inner Mitochondrial Membrane
Electron Transport Chain: Complex I and II
ROS generation is regulated and maintained at moderate levels necessary...
The Supercomplexes in the Crista Membrane
Overview of Fatty Acid Metabolism
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
Electron Transport Chain: Complex III and IV

