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A scanning electron microscope study of the developing rat secondary palate

P Cleaton-Jones

The South African Journal of Medical Sciences

|January 1, 1976

Summary

Cell surface degeneration in developing rat palates precedes fusion. Microvilli loss and cell separation occur before the palate fuses, suggesting a crucial role in this developmental process.

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Area of Science:

Developmental biology
Oral biology
Histology

Background:

Palatal fusion is a critical developmental process in mammals.
Understanding the cellular mechanisms of palatal fusion is essential for identifying causes of cleft palate.

Purpose of the Study:

To examine the surface changes of developing secondary palatal shelves and palate in Wistar rats.
To investigate the cellular events occurring on the medial aspect of palatal shelves prior to fusion.

Main Methods:

Scanning electron microscopy (SEM) was used to visualize the surface morphology of developing rat palates.
Observations were made at different time points during palatal development, starting from the 16th day post-insemination.

Main Results:

Progressive degeneration of cell surfaces was observed on the medial aspect of the palatal shelves starting on day 16.
This degeneration involved loss of microvilli, indistinct intercellular boundaries, and the formation of intercellular clefts.
Palatal fusion was complete by day 18, with two distinct epithelial zones visible in the soft palate.

Conclusions:

Cell surface degeneration, characterized by microvilli loss and cell separation, appears to be a key event preceding secondary palate fusion in Wistar rats.
These observed changes may be essential for successful shelf adhesion and fusion during palate development.

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