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Precise sequence complementarity between yeast chromosome ends and two classes of just-subtelomeric sequences

R J Britten1

  • 1Division of Biology, California Institute of Technology, 101 Dahlia Avenue, Corona del Mar, CA 92625, USA. rbritten@etna.bio.uci.edu

Proceedings of the National Academy of Sciences of the United States of America
|May 30, 1998
PubMed
Summary

The terminal regions of yeast chromosomes share extensive sequence similarity, suggesting a history of exchange. These subtelomeric sequences form distinct left and right classes, with specific repeats at chromosome ends.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Yeast Genomics

Background:

  • Chromosome ends in Saccharomyces cerevisiae (yeast) possess unique structural and sequence features.
  • Telomeres and subtelomeric regions play crucial roles in chromosome stability and function.

Purpose of the Study:

  • To investigate the sequence organization and relationships within the terminal regions of Saccharomyces cerevisiae chromosomes.
  • To identify patterns of sequence similarity and potential exchange between chromosome ends.

Main Methods:

  • Comparative sequence analysis of the terminal 20 kb of all Saccharomyces cerevisiae chromosomes.
  • Examination of both direct and reverse complement sequence similarities.
  • Identification and classification of repeated and unique sequences within subtelomeric regions.

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Main Results:

  • Universal blocks of precise sequence similarity exist between terminal regions of different yeast chromosomes.
  • Left and right terminal regions exhibit distinct patterns: reverse complements between left/right, direct similarity within left/right.
  • Subtelomeric sequences fall into two main classes, characterized by Y' or X2 repeats adjacent to telomeric repeats.
  • Extensive history of sequence exchange or duplication between terminal regions is inferred.

Conclusions:

  • The terminal regions of yeast chromosomes are not isolated but are highly interconnected through sequence similarity and exchange.
  • Distinct classes of subtelomeric repeats (Y' and X2) are conserved across multiple chromosome ends.
  • Sequence dynamics in subtelomeric regions contribute to genome evolution and stability.