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Updated: Jul 31, 2026
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Microfabrication of Nanoporous Gold Patterns for Cell-material Interaction Studies
Published on: July 15, 2013
Labeling with nanogold and undecagold: techniques and results
1Brookhaven National Laboratory, Biology Department, Upton, NY 11973, USA. hainfeld@genome1.bio.bnl.gov
Scanning Microscopy. Supplement
|January 1, 1996
Summary
New gold cluster compounds offer superior microscopy labeling. These smaller, covalently attached probes enhance tissue penetration and labeling density for diverse molecular targets.
Area of Science:
- Nanotechnology
- Bioconjugation Chemistry
- Microscopy
Background:
- Traditional colloidal gold probes have limitations in tissue penetration and labeling density.
- Development of novel labeling agents is crucial for advanced biological imaging.
Purpose of the Study:
- To introduce and characterize novel gold cluster compounds for enhanced microscopy labeling.
- To demonstrate the versatility of these gold probes for various biomolecules.
Main Methods:
- Covalent attachment of gold cluster compounds to antibodies and other probe molecules.
- Synthesis of novel fluorescent-gold conjugates.
- Evaluation of probe size, tissue penetration, and labeling density.
- Correlative fluorescence and electron microscopy imaging.
Main Results:
- Gold cluster probes are smaller than conventional colloidal gold conjugates.
- Demonstrated improved tissue penetration and higher labeling densities.
- Successfully created probes with peptides, nucleic acids, lipids, and drugs.
- Developed a fluorescent-gold conjugate enabling correlative microscopy.
Conclusions:
- Gold cluster compounds represent a significant advancement in gold labeling for microscopy.
- These novel probes offer enhanced performance and broader applicability in biological research.
- The fluorescent-gold conjugate facilitates multi-modal imaging for ultrastructural localization studies.
