Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

New nuclear functions for calmodulin

N Agell1, R Aligué, V Alemany

  • 1Department of Cell Biology, Faculty of Medicine, University of Barcelona, Spain.

Cell Calcium
|May 28, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Search for long-lived doubly charged Higgs bosons in pp collisions at sqrt[s] = 1.96 TeV.

Physical review letters·2005
Same author

Measurement of B(t --> Wb)/B(t--> Wq) at the collider detector at fermilab.

Physical review letters·2005
Same author

Search for new physics using high-mass tau pairs from 1.96 TeV pp collisions.

Physical review letters·2005
Same author

Multiple primary digital apocrine sweat gland carcinosarcoma in a cat.

The Veterinary record·2005
Same author

Measurement of the W(+)W(-) production cross section in pp collisions at square root[s]=1.96 TeV using dilepton events.

Physical review letters·2005
Same author

Search for anomalous kinematics in tt dilepton events at CDF II.

Physical review letters·2005
Same journal

Calorie restriction modulates beta cell IP<sub>3</sub>R activity to regulate Ca<sup>2+</sup> homeostasis and cell network connectivity.

Cell calcium·2026
Same journal

Spatially multiplexed stimulation reveals nonlinear calcium signaling in platelets.

Cell calcium·2026
Same journal

Calcium handling properties and arrhythmia vulnerability of cardiomyocytes from dystrophin-deficient mdx mice.

Cell calcium·2026
Same journal

Molecular actors of the Calcium signaling in the endothelial-to-mesenchymal transition: poorly explored therapeutic targets.

Cell calcium·2026
Same journal

High-throughput quantitation of pathogen-induced calcium signals captured through live-cell fluorescence microscopy.

Cell calcium·2026
Same journal

A model for the complete sequence of the human voltage-gated calcium channel (Ca<sub>V</sub>1.2) in a lipid bilayer.

Cell calcium·2026
See all related articles

Nuclear calmodulin (CaM) plays a crucial role in cell cycle progression by regulating cyclin-dependent kinases. This study identifies new CaM-binding proteins involved in transcription, splicing, and ribosomal assembly.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Nuclear calmodulin (CaM) is a calcium-binding protein with known roles in cellular signaling.
  • The precise functions of nuclear CaM in cell proliferation and gene expression remain incompletely understood.

Purpose of the Study:

  • To elucidate novel functions of nuclear calmodulin (CaM) in proliferating cells.
  • To identify new nuclear CaM-binding proteins and their roles in cellular processes.

Main Methods:

  • Utilized CaM inhibitors to assess effects on cell cycle kinases.
  • Employed CaM-affinity chromatography to identify binding proteins.
  • Performed phage display analysis in fission yeast to screen for CaM-binding proteins.

Related Experiment Videos

Main Results:

  • CaM inhibition blocked cyclin-dependent protein kinases 4 (cdk4) and 2 (cdk2) activity, impacting G1 progression and DNA replication.
  • Identified La/SSB and SAP145 as new nuclear CaM-binding proteins, suggesting CaM involvement in transcription and pre-mRNA splicing.
  • Discovered nucleolar-ribosomal proteins binding to CaM in yeast, indicating a role in ribosomal assembly/function.

Conclusions:

  • Nuclear CaM is a key regulator of cell cycle progression through modulation of cdk4 and cyclin D.
  • CaM is implicated in diverse nuclear processes including transcription, pre-mRNA splicing, and ribosome biogenesis.
  • These findings expand the known functional repertoire of nuclear calmodulin.