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Effects of semen components on ovulation and fertilization
1Institute for Reproductive Medicine, Veterinary School of Hannover, Germany.
Journal of Reproduction and Fertility. Supplement
|January 1, 1997
Summary
Seminal plasma infusion in pigs advances ovulation when administered early in estrus, enhancing the potential for successful fertilization. This effect is localized to the reproductive tract and mediated by specific seminal components.
Area of Science:
- Reproductive Biology
- Animal Science
- Veterinary Medicine
Background:
- Transcervical infusion of seminal plasma in pigs during estrus can advance ovulation.
- Understanding the precise mechanisms and optimal timing is crucial for potential applications in artificial insemination (AI).
Purpose of the Study:
- To investigate the effect of transcervical seminal plasma infusion on ovulation timing in pigs.
- To determine if the effect is systemic or local and identify active components.
Main Methods:
- Utilized a surgical model for single uterine horn infusion in pigs.
- Employed transcutaneous sonography for ovarian monitoring.
- Administered seminal plasma or PBS transcervically at estrus onset and monitored ovulation and LH profiles.
Main Results:
- Seminal plasma infusion advanced ovulation by 8–14 hours in the ipsilateral ovary compared to the contralateral ovary.
- The effect was dependent on early estrus infusion and more pronounced in gilts with longer estrus-to-ovulation intervals.
- No significant changes in LH profiles were observed, suggesting a local mechanism.
- Seminal plasma administered 24 hours after estrus onset was ineffective.
Conclusions:
- Seminal plasma acts locally within the female genital tract to advance ovulation in pigs.
- The ovulatory advancement is mediated by low molecular mass protein fractions and seminal estrogens.
- Early estrus administration is critical for efficacy, with potential applications in improving AI outcomes.