Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Developments in transgenic techniques in pigs

M B Nottle1, H Nagashima, P J Verma

  • 1BresaGen Limited, Adelaide, Australia.

Journal of Reproduction and Fertility. Supplement
|January 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multiple ovulation and embryo transfer in sheep: Effects of embryo developmental stage and quality on viability in vivo under farm conditions.

Australian veterinary journal·2022
Same author

Supplementation of culture medium with L-carnitine improves the development and cryotolerance of in vitro-produced porcine embryos.

Reproduction, fertility, and development·2017
Same author

Field fertility of liquid stored and cryopreserved flow cytometrically sex-sorted stallion sperm.

Equine veterinary journal·2016
Same author

Proteomic characterization and cross species comparison of mammalian seminal plasma.

Journal of proteomics·2013
Same author

Quercetin improves the postthaw characteristics of cryopreserved sex-sorted and nonsorted stallion sperm.

Theriogenology·2013
Same author

Dimethyl formamide improves the postthaw characteristics of sex-sorted and nonsorted stallion sperm.

Theriogenology·2013

Precise pig genome editing is needed, as current random DNA insertion methods are limited. Future techniques may enable targeted gene insertion using nuclear transfer or in situ modifications.

Area of Science:

  • Animal Genetics
  • Genomic Engineering
  • Biotechnology

Background:

  • Current pig genome manipulation relies on pronuclear microinjection, a method with limitations.
  • Pronuclear microinjection results in random DNA integration, hindering precise genetic modification and gene targeting.

Purpose of the Study:

  • To explore advanced methods for precise genome editing in pigs.
  • To overcome the limitations of random DNA insertion in pig genetic engineering.

Main Methods:

  • Discusses the limitations of pronuclear microinjection for targeted gene insertion.
  • Highlights the success of embryonic stem (ES) cell and homologous recombination in mice.
  • Proposes nuclear transfer for reprogramming differentiated cells as a potential alternative in pigs.

Related Experiment Videos

Main Results:

  • Embryonic stem cells, crucial for precise editing in mice, are not yet isolated in pigs.
  • Nuclear transfer offers a potential pathway for generating precise genetic modifications in pigs.
  • Future development of in situ genome editing methods is anticipated.

Conclusions:

  • Precise genomic site-directed insertion is essential for improved pig genetic engineering.
  • Nuclear transfer and potential in situ methods may replace current techniques for targeted pig genome modification.