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Related Experiment Videos

Advances in gender preselection in swine

L A Johnson1

  • 1Germplasm and Gamete Physiology Laboratory, US Department of Agriculture, Beltsville, MD 20705, USA.

Journal of Reproduction and Fertility. Supplement
|January 1, 1997
PubMed
Summary

Sperm sexing technology allows for the selection of desired offspring sex in livestock and humans by separating X- and Y-chromosome sperm based on DNA content. This validated method achieves 85-90% accuracy for desired sex ratios in various species.

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Area of Science:

  • Reproductive Biology
  • Animal Science
  • Genetics

Background:

  • Gender preselection in livestock and humans is achievable through sperm separation techniques.
  • Existing methods isolate X- and Y-chromosome bearing spermatozoa based on DNA content differences.

Purpose of the Study:

  • To validate and improve sperm sexing technology for livestock and human applications.
  • To assess the efficiency and applicability of flow cytometry-based sperm separation.

Main Methods:

  • Spermatozoa are stained with Hoechst 33342, binding DNA proportionally.
  • Modified flow cytometry and cell sorting separate sperm based on DNA content differences.
  • Separated sperm are used for insemination (intra-tubal, deep-uterine) or in vitro fertilization (IVF).

Main Results:

  • The USDA-Beltsville Sperm Sexing Technology has produced over 300 offspring across species.
  • Achieved desired sex ratios of 85-90% for one sex.
  • Offspring in cattle, swine, and rabbits show normal morphology and reproductive function through two generations.

Conclusions:

  • Sperm sexing technology is validated for producing offspring of the desired sex in farm animals and humans.
  • Current limitations include optimizing artificial insemination techniques for small sperm populations.
  • Further improvements in sexing speed are crucial for broader industry adoption.

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