Aneuploidy induced by dimethylarsinic acid in mouse bone marrow cells

We investigated the cytogenetic effects of dimethylarsinic acid (DMA), which is the major metabolite of inorganic arsenic compounds, on mouse bone marrow cells after a single intraperitoneal injection to mice. DMA increased mitotic indices significantly at 16, 24 and 48 h after injection, and prolonged the average generation time 1.5 h at the 24 h. These results suggest that DMA may cause mitotic arrest in vivo as well as in vitro. However the activity of mitotic arrest induced by DMA was much weaker than that induced by colchicine. Metaphase cells obtained after administration of DMA without colchicine pretreatment were morphologically normal except for chromosome number, which varied by stage from the prophase to the telophase in M phase as seen after administration of saline. DMA significantly induced aneuploids. The frequencies of euploids with DMA and saline treatment were 55.1 and 94.0%, respectively, and in DMA treatment hyperploids with 1 or 2 extra chromosomes were over 80% of all aneuploids. These results suggest that aneuploidy induced by DMA might be associated with carcinogenicity of arsenic.