Adaptation of the cytokinesis-block technique for genotoxicity assay on L929 cell line

The in vitro micronucleus assay was used as a method for evaluation of chromosomal damage in L929 cells. Known genotoxins were used to induce micronuclei: bleomycin the direct acting agent, and cyclophosphamide the substance which requires metabolic activation. The cytokinesis-block technique and the use of exogenous metabolizing system is precisely described.