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Preimplantation diagnosis by FISH: the Rambam experience
D Manor1, D Stein, J Itskovitz-Eldor
1Department of Obstetrics and Gynecology, Rambam Medical Center, Haifa, Israel.
Journal of Assisted Reproduction and Genetics
|May 30, 1998
Summary
Fluorescence in situ hybridization (FISH) effectively detects aneuploidy in preimplantation genetic diagnosis, showing 87% accuracy for autosomes and 97% for gonosomes. This method is suitable for specific in vitro fertilization cases, including fast-dividing embryos.
Area of Science:
- Reproductive Medicine
- Genetics
- Embryology
Background:
- Preimplantation genetic diagnosis (PGD) aims to improve in vitro fertilization (IVF) outcomes.
- Aneuploidy screening is crucial for selecting viable embryos for transfer.
Purpose of the Study:
- To summarize experience with fluorescence in situ hybridization (FISH) for PGD.
- To evaluate FISH efficiency in detecting chromosomal abnormalities in embryos.
Main Methods:
- FISH analysis was performed on blastomeres from 73 embryos across 29 IVF cycles.
- Indications included advanced maternal age, X-linked diseases, poor embryo quality, and recurrent IVF failure.
- Probes for chromosomes X, Y, 13, 18, and 21 were used for ploidy detection.
Main Results:
- Clear FISH signals were obtained in 87% of analyzed blastomeres.
- Two pregnancies resulted in two healthy births.
- Normal FISH signals were observed in 57% of fast-dividing embryos and in 50% and 11.4% of embryos with unequal pronuclei size.
Conclusions:
- FISH demonstrates high efficiency for aneuploidy detection (87% autosomes, 97% gonosomes).
- PGD using FISH is beneficial for selected IVF cases, including those with fast-dividing or morphologically atypical embryos.