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Immunolocalization of stanniocalcin in human kidney
1Department of Physiology, Faculty of Dentistry and Medicine, University of Western Ontario, London, Canada.
Molecular and Cellular Endocrinology
|May 30, 1998
Summary
Human stanniocalcin (STC) is a hormone regulating mineral metabolism. This study identified human STC as a glycosylated dimer in kidney cells, confirming its role in renal phosphate transport.
Area of Science:
- Biochemistry
- Endocrinology
- Nephrology
Background:
- Stanniocalcin (STC) is a mammalian hormone involved in mineral metabolism.
- Its role in renal phosphate transport is suggested but not fully elucidated in humans.
Purpose of the Study:
- To characterize human tissue-derived STC.
- To determine the physical and chemical properties of human STC.
- To localize STC within the human kidney.
Main Methods:
- Characterization of purified human STC.
- Comparison with baculovirus-expressed STC.
- Immunohistochemical localization in human kidney tissue.
Main Results:
- Human STC is a glycosylated, disulfide-linked dimer.
- Its properties are similar to baculovirus-expressed STC.
- STC was localized to principal and alpha-intercalated cells in the distal nephron.
Conclusions:
- This is the first homologous demonstration of STC protein and its cellular localization in human tissue.
- The findings support STC's role in human mineral and renal phosphate metabolism.