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Primary epitopes of chicken egg yolk antibodies to peptidophosphogalactomannan
B A Tuekam1, S J Bonetti, J E Gander
1Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville 32611-0700, USA.
Clinical and Diagnostic Laboratory Immunology
|May 30, 1998
Abstract:
Egg yolks from hens immunized with peptidophosphogalactomannan (pPGalMan(ii)), which contains 10 phosphocholine diester residues and is secreted by Penicillium fellutanum, contain antibodies against 5-O-beta-D-galactofuranosyl epitopes. These epitopes were the only significant determinants in pPGalMan(ii). Approximately 60-fold less pPGalMan(ii) (1.6 microM galactofuran chains) was required for 50% inhibition than galactofurano-oligosaccharides or pPGalMan containing two galactofuranosyl residues per chain.