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Related Experiment Videos

Nitrosothiol quantification in human plasma

R K Goldman1, A A Vlessis, D D Trunkey

  • 1Department of Surgery, Oregon Health Sciences University, Portland 97201-3098, USA.

Analytical Biochemistry
|May 30, 1998
PubMed
Summary
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A new high-pressure liquid chromatography (HPLC) assay accurately measures nitrosothiol in biological samples. This sensitive method quantifies picomole levels using catalytic reduction and azo dye formation for precise nitrosothiol detection.

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Environmental Science

Background:

  • Nitrosothiols are important biological molecules implicated in various physiological and pathological processes.
  • Accurate quantification of nitrosothiols in biological matrices is crucial for understanding their roles.
  • Existing methods for nitrosothiol detection may lack sensitivity or specificity.

Purpose of the Study:

  • To develop a sensitive and accurate assay for measuring picomole quantities of nitrosothiol in biological samples.
  • To establish a reliable method for quantifying nitrosothiol concentration in complex biological matrices.

Main Methods:

  • Development of a high-pressure liquid chromatography (HPLC) assay.
  • Utilizing catalytic reduction of nitrosothiol by mercuric cation (Hg2+).

Related Experiment Videos

  • Quantification of a stable azo dye formed from released nitrogen oxide, sulfanilamide (SA), and N-(1-napthyl)ethylenediamine (NNED) via reversed-phase HPLC.

    • Main Results:

    • The assay is highly sensitive, detecting approximately 3 pmol of nitrosothiol (approximately 10(-1) microM).
    • A reference sample strategy effectively subtracts interference from nitrite and atmospheric nitrogen oxides.
    • The method provides accurate quantification of nitrosothiol in biological samples.

    Conclusions:

    • The developed HPLC assay offers a sensitive and accurate means for measuring nitrosothiol concentrations in biological samples.
    • This assay is valuable for research requiring precise quantification of nitrosothiols.
    • The method addresses limitations of previous techniques by minimizing interference and enhancing sensitivity.