Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Defining the interactions between intermediate filaments and desmosomes

E A Smith1, E Fuchs

  • 1Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois 60637, USA.

The Journal of Cell Biology
|June 12, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Observation of tt[over ¯] Production in Pb+Pb Collisions at sqrt[s_{NN}]=5.02  TeV with the ATLAS Detector.

Physical review letters·2025
Same author

Search for Dark Matter Produced in Association with a Dark Higgs Boson in the bb[over ¯] Final State Using pp Collisions at sqrt[s]=13  TeV with the ATLAS Detector.

Physical review letters·2025
Same author

Search for Magnetic Monopole Pair Production in Ultraperipheral Pb+Pb Collisions at sqrt[s_{NN}]=5.36  TeV with the ATLAS Detector at the LHC.

Physical review letters·2025
Same author

Simultaneous Unbinned Differential Cross-Section Measurement of Twenty-Four Z+jets Kinematic Observables with the ATLAS Detector.

Physical review letters·2025
Same author

Disentangling Sources of Momentum Fluctuations in Xe+Xe and Pb+Pb Collisions with the ATLAS Detector.

Physical review letters·2025
Same author

Search for Light Long-Lived Particles in pp Collisions at sqrt[s]=13  TeV Using Displaced Vertices in the ATLAS Inner Detector.

Physical review letters·2024

Desmoplakin and plakoglobin anchor desmosomes to intermediate filaments, with plakophilin 1 reinforcing this link during epidermal cell differentiation for increased desmosome stability.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Dermatology

Background:

  • Desmoplakin (DP), plakoglobin (PG), and plakophilin 1 (PP1) are non-transmembrane desmosomal proteins.
  • These proteins are candidates for linking intermediate filaments (IFs) to desmosomes.
  • Understanding these interactions is crucial for epidermal cell structure and stability.

Purpose of the Study:

  • To elucidate the molecular mechanisms by which DP, PG, and PP1 interact with desmosomes and intermediate filaments.
  • To identify the specific domains and interactions responsible for anchoring IFs to desmosomes.
  • To model the dynamic assembly and reinforcement of desmosomes during epidermal differentiation.

Main Methods:

  • Deletion and site-directed mutagenesis of desmoplakin.

Related Experiment Videos

  • In vitro overlay assays to assess protein-protein interactions.
  • Analysis of interactions between desmosomal proteins and epidermal keratins.

    • Main Results:

    • A small N-terminal region of DP (86 residues) is sufficient for desmosome targeting.
    • DP, PG, and PP1 interact with desmosomal cadherins (Dsc1a, Dsg1) and with each other.
    • DP, PG, and PP1 associate with epidermal keratins, particularly type II keratins, with varying affinities.

    Conclusions:

    • DP and PG form an initial scaffold anchoring desmosomal cadherins to keratin IFs in dividing epidermal cells.
    • PP1 is added during differentiation, reinforcing the desmosome-IF linkage and enhancing plaque stability.
    • These findings provide a model for the dynamic assembly and stabilization of desmosomes in the epidermis.