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Contamination of mouse-adapted influenza virus with Sendai virus
K Takimoto1, K Nakayama, M Yabe
1Division of Experimental Animal Research, National Institute of Infectious Diseases, Tokyo, Japan.
Abstract:
In our laboratory animal facility, Sendai virus (HVJ) contamination occurred in a negative flow rack used for experimental infection with 4 strains of mouse-adapted influenza virus (Inf.V). Anti-HVJ antibody (Ab) was detected in 35/42 mice in the rack. To specify the strain of Inf.V contaminated with HVJ, experimental infection was performed by using A, B and D strains of Inf.V in each vinyl isolator. Anti-HVJ Ab was detected in all mice infected with A strain at day 28 post-infection. As a result of experimental infection with A strain of Inf.V which was treated with anti-HVJ mouse serum, the virus suspension was determined not to contain HVJ and allowed for experimental use in our facility, Since then, HVJ contamination has not occurred in our facility.
Insights
Sendai virus (HVJ) contamination in a lab rack was traced to a specific influenza virus strain (Inf.V A). Treating the virus with anti-HVJ serum neutralized contamination, ensuring safe experimental use and preventing further spread.
Area of Science:
- Veterinary Virology
- Laboratory Animal Science
- Infectious Disease Research
Background:
- Sendai virus (HVJ) contamination poses a significant risk in laboratory animal facilities.
- Contamination was identified in a flow rack used for influenza virus (Inf.V) experiments.
- Detection of anti-HVJ antibodies in mice indicated widespread exposure.
Purpose of the Study:
- To identify the specific influenza virus strain responsible for Sendai virus (HVJ) contamination.
- To develop and validate a method to eliminate HVJ contamination from experimental materials.
- To ensure the safety and integrity of laboratory animal experiments.
Main Methods:
- Experimental infection of mice with different strains of mouse-adapted influenza virus (Inf.V).
- Detection of anti-HVJ antibodies in experimentally infected mice.
- Treatment of suspected contaminated virus suspensions with anti-HVJ mouse serum.
Main Results:
- Anti-HVJ antibodies were detected in 35/42 mice exposed to the contaminated rack.
- All mice infected with Inf.V strain A showed anti-HVJ antibodies by day 28 post-infection.
- Treatment with anti-HVJ serum rendered the Inf.V A suspension free of HVJ, permitting its use.
Conclusions:
- Influenza virus A strain was identified as the source of HVJ contamination.
- Treatment with specific antiserum is an effective method to eliminate HVJ contamination.
- Implementing this method successfully prevented further HVJ contamination in the facility.