[Reproduction and synthesis of proteins from human adenovirus serotype 4]

Multiplication and protein synthesis of type 4 human adenovirus (Ad 4) were investigated. Immunofluorescence patterns (diffuse and distinct spot nuclear fluorescence) of hexon and process ad 4 were different when monoclonal antibodies to these proteins were used. Mature antigen-active capsid and core proteins were detected by Western blotting at different periods postinfection. Ad 4 effectively multiplied in human cells HeLa and A549, less so in monkey cells (Vero), and poorly in murine cells (L, BF(Balb). The synthesis of structural virus proteins was suppressed in murine cells and little changed in simian cells. DNA-binding protein has molecular weight of 50,000 daltons at Ad 4 infection of Vero cells as against 72,000 daltons in infected L and HeLa cells.